Sr. Research Associate, Delivery

AskBio Inc. a wholly owned and independently operated subsidiary of Bayer AG is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular central nervous system cardiovascular and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure limb-girdle muscular dystrophy multiple system atrophy Parkinsons disease and Pompe disease. AskBios gene therapy platform includes Pro10 an industry-leading proprietary cell line manufacturing process and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park North Carolina and European headquarters in Edinburgh Scotland the company has generated hundreds of proprietary capsids and promoters several of which have entered pre-clinical and clinical testing.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform peoples lives.

Our principles:

• Advance innovative science by pushing boundaries.

• Bring transformative therapeutics to patients in need.

• Provide an environment for employees to reach their fullest potential.

Our values:

• Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

• Cultivate Collaboration. Strive to be the best teammate actively listen openly communicate and embrace diverse points of view.

• Embrace Responsibility. We are humbled by the enormity of our hold a relentless commitment to advance science and clinical outcomes for our patients families and caregivers.

• Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

• Act with Uncompromising Integrity. Be honest transparent and committed to doing whats right in every situation. Make clear commitments and follow through.

Position Summary

AskBio is seeking a highly motivated enthusiastic and collaborative Senior Research Associate to support the development and discovery of next-generation gene therapies utilizing state-of-the-art non-viral delivery technologies. The Senior Research Associate will work directly with the non-viral delivery team to conduct experiments that advance our preclinical therapeutic pipeline.

The ideal candidate will have hands-on experience with standard molecular biology techniques including: (1) isolation of DNA RNA and protein from cells and tissues; and (2) quantitative PCR (qPCR) RT-PCR ddPCR ELISA SDS-PAGE and Western blot. Prior experience with research-grade lipid nanoparticle (LNP) formulation is preferred.

This position is based in Morrisville North Carolina.

Job Responsibilities

• Perform plasmid purification genomic DNA extraction RNA extraction and PCR-based assays (PCR qPCR ddPCR)

• Conduct small-scale LNP formulation and analytical characterization for early research and preclinical in vivo and in vitro studies

• Perform in vitro cell-based assays including plasmid transfection AAV transduction LNP transfection and primary cell culture

• Analyze data and effectively communicate results through formal reports and presentations for internal and external stakeholders

• Develop detailed experimental protocols and maintain an electronic laboratory notebook in accordance with good documentation and data integrity practices

• Maintain inventory and order reagents and materials to support timely project execution

• Ensure laboratory areas are clean organized and adequately stocked with necessary equipment and supplies

Minimum Requirements

• Bachelors degree in molecular biology Biochemistry or a related field with 5 years of research experience; OR a Masters degree in molecular biology Biochemistry or a related field with 2 years of research experience

• Hands-on experience with molecular biology techniques including DNA/RNA isolation SDS-PAGE qPCR and Western blot

• Clear understanding of in vivo gene therapy approaches including adeno-associated virus (AAV) and lipid nanoparticles (LNP)

• Experience with cell line maintenance including primary cell culture

• Strong technical laboratory skills with the ability to independently execute protocols and experiments

• Ability to manage multiple projects simultaneously and work independently in a fast-paced collaborative environment

• Ability to follow established molecular biology SOPs with minimal supervision

• Highly organized self-motivated and able to work both independently and within cross-functional teams

• Excellent written and verbal communication skills including accurate documentation practices

• Proficiency in Microsoft Office Suite (Word Excel PowerPoint)

• Strong problem-solving skills and sound scientific judgment

Preferred Education Experience and Skills

• Experience with research-grade LNP formulation and analytical characterization

• Experience with next-generation sequencing (NGS)

• Understanding of statistics and data analysis/interpretation

• Experience using SnapGene and GraphPad

AskBio Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race color religion gender sexual orientation gender identity national origin age disability veteran status or any other protected status prohibited under Federal State or local laws. All employment decisions are based on valid jobrelated requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website you may request a reasonable accommodation to express interest in a specific opening by calling us at or sending us an email at .

Agencies: Please do not contact any employee at AskBio about this requisition. Any resume submitted by a recruitment agency to any employee at AskBio through any medium will be deemed the sole property of AskBio unless the agency was engaged by AskBio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with AskBio prior to any search engagement. If a candidate who was submitted outside of the AskBio agency process is hired by AskBio no fee or payment of any kind will be paid to the agency.