Research Laboratory Technician

Job Description

This position is supported by a newly funded NIGMS Maximizing Investigators Research Award (MIRA R35) to Dr. James Weger-Lucarelli which investigates how viral and host factors shape regulated cell death (RCD) and modulate virus replication with a focus on single- and multicellular 3D tissue-relevant systems. We are seeking a highly motivated enthusiastic postdoc with hands-on expertise in building and characterizing 3D multicellular tissue models including hiPSC-derived and primary-cell co-culture/organoid systems to lead studies defining how spatial and multicellular context shapes cell death pathway engagement extracellular danger signaling (DAMPs/EVs) and replication of alphaviruses (Semliki Forest virus) and orthoflaviviruses (Zika virus).
The ideal candidate will have direct experience establishing multicellular 3D organoid or co-culture systems and differentiating cells into relevant cell types (e.g. M1- and M2-macrophages/microglia) alongside a solid conceptual understanding of virology immunology or host-pathogen interactions. The candidate will also apply molecular cellular and imaging-based approaches including flow cytometry qPCR western blotting ELISA and confocal microscopy to quantify RCD and characterize virus-host interactions and will collaborate with computational and imaging collaborators on single-cell RNA sequencing datasets generated from these models.
A positive attitude and a willingness to face uncomfortable situations with the goal of learning are crucial to success in this position. The PI will work directly with the candidate and seek to help the candidate develop their career in the trajectory that best suits their interests. Duties will involve performing in vitro procedures and training in BSL2 and BSL3 labs. Responsibilities also include data analysis report and manuscript preparation and compliance with all safety regulations. Finally the candidate is expected to train and assist graduate and undergraduate students in their experiments in collaborative projects.

Required Qualifications

Ph.D. in Cell Biology Immunology Virology Pathology Biomedical Sciences or a closely related field.
Hands-on experience establishing maintaining and characterizing multicellular 3D tissue models (organoids spheroids or co-cultures) derived from primary and/or hiPSC-derived cells.
Experience with hiPSC culture maintenance and directed differentiation protocols.
Humility: The desire to constantly learn and take feedback from everyone.
Leadership: The desire to lead and complete projects and take ownership of successes and failures.
Teamwork: The desire to work closely with teammates towards the overall goal of the lab.
Initiative: Someone who strongly favors action over inaction.
Molecular and protein biology skills including experience with flow cytometry RT-qPCR western blotting and/or ELISA.
Substantial experience independently organizing and conducting complex procedures and experiments with minimal supervision.
Should be enthusiastic and dedicated have the ability and interest in learning new techniques including virus handling and BSL2/BSL3 procedures (training provided).

Preferred Qualifications

Prior experience working directly with viruses or in an infectious disease research setting.
Experience with confocal or spinning-disk confocal microscopy and image-based analysis.
Experience with single-cell RNA sequencing (scRNA-seq) library preparation and/or analysis.
Experience with immunocytochemical staining in vivo/animal procedures or CRISPRi/CRISPRa perturbation approaches.

Pay Band

3; Salary

Overtime Status

Non-Exempt: Eligible for overtime

Appointment Type

Restricted

Salary Information

Commensurate with Experience

Hours per week

40

Review Date

08/21/2026

Additional Information

The successful candidate will be required to have a criminal conviction check.

About Virginia Tech

Dedicated to its mottoUt Prosim(That I May Serve) Virginia Tech pushes the boundaries of knowledge by taking a hands-on transdisciplinary approach to preparing scholars to be leaders and problem-solvers. A comprehensive land-grant institution that enhances the quality of life in Virginia and throughout the world Virginia Tech is aninclusive communitydedicated to knowledge discovery and creativity. The university offers more than 280 majors to a diverse enrollment of more than 36000 undergraduate graduate and professional students in eightundergraduate colleges aschool of medicine aveterinary medicinecollegeGraduate School andHonors College.The university has a significant presence across Virginia including Blacksburg the greater Washington D.C. area the Health Sciences and Technology Campus in Roanoke sites in Newport News and Richmond and numerousExtension officesandresearch institutes.A leading global research institution Virginia Tech conducts more than $650 million in research annually.

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